Journal: Cell
Article Title: iPSCs from a hibernator provide a platform for studying cold adaptation and its potential medical applications
doi: 10.1016/j.cell.2018.03.010
Figure Lengend Snippet: Key Resources Table
Article Snippet: Using primers specific for the expression of 3 pluripotent marker genes (Pou5f1, Nanog, Sox2) and 10 differentiation markers (Dppa5, Pdx1, Nestin, Sox17, Otx2, Tp63, Foxa2, Gata4, Afp, Brachyury) we confirmed by qRT-PCR the pluripotency of the GS iPSC colonies (data not shown). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Poly-E-T (1:1000) AdipoGen Cat# AG-20B-0020-C100 Mouse monoclonal anti-TUBB3 (1:1000) R&D Systems Cat# MAB1195 Mouse monoclonal anti-OCT3/4 (1:500) Santa Cruz Cat# sc-5279 Rabbit polyclonal anti-NANOG (1:500) Cosmo Bio Cat# RCAB0004P-F Mouse monoclonal anti-ALBUMIN (1:500) Cedarlane Cat# CL2513A Rabbit monoclonal anti-HNF4α (1:500) Cell Signaling Cat# 3113S Rabbit polyclonal anti-DESMIN (1:500) Thermo Fisher Cat# RB9014P1 Mouse anti-TNNT (1:500) Thermo Fisher Cat# MS295P0 Rabbit polyclonal anti- Δ2-T (1:1000) Millipore Cat# AB3203 Rabbit polyclonal anti-Poly-E-T (1:2000) AdipoGen Cat# AG-25B-0030-C050 Rabbit polyclonal anti-TUBB3 (1:2000) BioLegend Cat# 802001 Mouse monoclonal anti-TUBA (1:200) Sigma Cat# F2168 Rabbit monoclonal anti-LAMP1 (1:1000) Cell Signaling Cat# 9091 Rabbit polyclonal anti-VCP (p97) (1:1000) Cell Signaling Cat# 2648 Mouse monoclonal anti-VCP (p97) (1:5000) Fitzgerald Cat# 10R-P104a Mouse monoclonal anti-PSMB2 (1:1000) Santa Cruz Cat# SC-515066 Mouse monoclonal anti-PSMB5 (1:1000) Santa Cruz Cat# SC-393931 Mouse monoclonal anti-HSP60 (1:1000) Santa Cruz Cat# 4868 Mouse monoclonal anti-HSP70 (1:1000) Santa Cruz Cat# sc-7298 Mouse monoclonal anti-HSP90 (1:1000) Santa Cruz Cat# sc-13119 Rabbit monoclonal anti-β-actin (1:1000) Cell Signaling Cat# 4970 Rabbit polyclonal anti-cleaved Caspase-3 (1:500) Cell Signaling Cat# 9661 Chicken polyclonal anti-GFP (1:1000) Aves Labs Cat# GFP-1020 Rabbit polyclonal anti-RBM3 (1:1000) Proteintech Cat# 14363-1-AP Rabbit polyclonal anti-CIRP (1:1000) Proteintech Cat# 10209-2-AP Chemicals, Peptides, and Recombinant Proteins Cell Culture Media Formulations & Reagents This paper N/A B27 supplement minus insulin Thermo Fisher Cat# A1895601 CHIR99021 Tocris Cat# 4423 IWP4 Tocris Cat# 5214 B27 supplement (complete) Thermo Fisher Cat# 17504044 RPMI Thermo Fisher Cat# 11875093 b-FGF R&D Systems Cat# 233-FB-025 HGF R&D Systems Cat# 294-HG-005 Hepatocyte Maintenance Media w/ Supplement Lonza Cat# MM250 Oncostatin M R&D Systems Cat# 295-OM-010 MG-132 (5 μM) Enzo Life Sci.
Techniques: Recombinant, Cell Culture, Protease Inhibitor, Lysis, SYBR Green Assay, In Vitro, Transfection, Imaging, Software